We provide end-to-end antibody development services, combining advanced biotechnology platforms with applied research expertise to accelerate your R&D pipeline.
We provide end-to-end antibody development services, combining advanced biotechnology platforms with applied research expertise to accelerate your R&D pipeline.
From discovery to proof of concept.
Accelerating therapeutic development.
Extending technical and scientific capabilities.
Supporting cutting-edge research.
As a specialized technology center, we bridge the gap between early-stage research and industrial application, supporting biotech, pharmaceutical and diagnostic companies in the generation and validation of high-value antibody-based tools with strong application and market potential.
Our integrated technology-driven approach reduces development risk, accelerates timelines and ensures scientific and technical excellence throughout every stage of the antibody discovery and validation process.
We provide expert guidance for the selection of the most suitable antibody-based solution and apply advanced discovery technologies to generate high-affinity, highly specific antibody candidates tailored to the desired application.
We lead the design of the optimal immunization strategies and the generation of suitable immunogens, from the design and production of conjugated peptides to the expression and purification of recombinant proteins in both prokaryotic and eukaryotic systems.
We offer comprehensive polyclonal antibody development services, from animal immunization and serum production to antibody purification and characterization. Depending on the application requirements, antibodies can be purified using different strategies, including total IgG purification, antigen-specific affinity purification, and customized purification workflows to reduce cross-reactivity and improve specificity.
Our processes are designed to deliver high-quality polyclonal antibodies optimized for research, diagnostic and industrial applications.
Hybridoma technology
We provide end-to-end monoclonal antibody development services based on hybridoma technology, from immunization and cell fusion to clone screening, selection and expansion. Using robust screening and validation strategies, we identify high-affinity and highly specific monoclonal antibodies tailored to the intended application. Selected clones can be further characterized, adapted to different production formats and used for scalable antibody production for research, diagnostic or industrial applications.
Phage display technology (scFv & VHH)
We offer antibody discovery services based on phage display technologies, including the generation and screening of both naïve and immune libraries. Our platforms include VHH libraries from camelids as well as scFv libraries derived from multiple species, including human, mouse, chicken, pig and cow. Through advanced selection and screening strategies (protein, cell, organoids or in vivo based), we identify antibody fragments with high affinity, specificity and optimal functional properties for research, diagnostic and therapeutic applications. Clients can inquire about the immune and naïve libraries currently available in our collection from different species, and may also provide their own custom libraries for screening and selection campaigns adapted to specific targets or discovery objectives
We provide antibody engineering services focused on the optimization and redesign of antibody molecules for specific research, diagnostic and therapeutic applications.
Our capabilities include the generation of bispecific antibodies, humanization, chimerization, class switching, and biosimilar development.
Using advanced molecular engineering and recombinant expression strategies, we tailor antibody formats and functionalities to achieve the desired performance, specificity, and developability profiles.
We provide scalable antibody production using mammalian expression systems suitable for research, preclinical and diagnostic applications.
We offer recombinant antibody expression services covering a wide range of antibody formats, including full-length monoclonal antibodies, antibody fragments, bispecific antibodies and engineered derivatives.
Production can be performed in hybridoma cells as well as mammalian expression systems such as CHO and HEK cells, using both transient and stable expression platforms depending on project scale and application requirements.
Our services include vector design and optimization, clone generation and selection, expression optimization, upstream process development and scalable antibody production.
Antibodies can be produced and purified for research, diagnostic or pre-industrial evaluation, with rigorous quality control to ensure consistency, purity and functional performance.
We provide flexible antibody production services using multiple cultivation formats, including shake flasks and high-density culture platforms, obtaining over 10L of supernatant per week.
Depending on the antibody format and production requirements, we optimize culture conditions to maximize yield, viability, and product quality.
Our capabilities also include adaptation of cell lines to serum-free media, optimization of culture media formulations, and refinement of production protocols to improve scalability and reproducibility. These strategies enable efficient small- to medium-scale antibody production for research, diagnostic and pre-clinical applications.
We provide advanced antibody purification services using fully controlled chromatography workflows performed on ÄKTA Pure and ÄKTA Go systems under temperature-controlled conditions at 4 °C to ensure antibody stability and product integrity.
Our purification capabilities include affinity chromatography using Protein A, Protein G or antigen-specific matrices, immobilized metal affinity chromatography (IMAC/Ni-NTA) for tagged recombinant proteins and antibody fragments, as well as size exclusion chromatography (SEC) for polishing, buffer exchange and aggregate removal.
Additional purification and polishing strategies can be implemented according to the specific antibody format and application requirements, ensuring high purity, reproducibility and optimal functional performance.
We perform comprehensive functional and analytical characterization to ensure antibody performance and reliability.
We provide advanced antibody interaction and biophysical characterization services using label-free technologies such as surface plasmon resonance (SPR) and biolayer interferometry (BLI) under optimized and standardized experimental conditions.
Our capabilities include affinity determination, kinetic analysis of antibody–antigen interactions, epitope binning, competition studies and binding specificity assessment for a wide range of antibody formats and targets.
Assays can be adapted to different experimental settings and analytical needs, providing detailed insight into molecular binding behavior and interaction profiles.
We provide comprehensive antibody characterization and testing services using a broad range of antibody-based assays performed under optimized and standardized experimental conditions to ensure reliability, reproducibility and high analytical quality.
Our capabilities include direct, indirect and competitive ELISA formats, cell-based ELISA (CELISA), Western blot analysis, flow cytometry–based assays, immunofluorescence and immunohistochemistry for antibody specificity and target recognition across multiple applications and sample types.
In addition, we offer assay development and optimization tailored to specific antibody formats, targets and experimental requirements, enabling robust and application-relevant characterization of antibody performance.
We provide comprehensive in vitro and in vivo functional characterization services to evaluate the biological activity, efficacy, and mechanism of action of antibody candidates across a wide range of applications and experimental models.
Our capabilities include cell-based functional assays, target engagement studies, efficacy evaluation in relevant disease models, as well as pharmacokinetic and pharmacodynamic (PK/PD) analyses and biodistribution studies to assess antibody exposure, tissue targeting, and in vivo behavior.
We also offer biomarker analysis services using both conventional ELISA platforms and multiplex technologies, including Luminex® and Olink®, enabling the simultaneous quantification of multiple analytes from limited sample volumes. In addition, we perform comprehensive immune monitoring studies, encompassing humoral and cellular immune response characterization, as well as allergy and hypersensitivity assessments.
Experimental strategies can be tailored to specific project objectives and antibody modalities, enabling integrated evaluation of functional performance, immunological impact, and therapeutic potential.
We provide diagnostic assay development services focused on the generation and optimization of customized immunodetection platforms for research and translational applications.
Our capabilities include the development of sandwich ELISA assays, custom multiplex immunoassays using bead-based Luminex xMAP technology for simultaneous detection of multiple analytes, and lateral flow immunoassay (LFIA) prototypes based on nanoparticle colorimetric detection.
Assay formats and analytical workflows can be tailored to specific biomarkers, sample types and performance requirements, supporting the development of sensitive, robust and application-oriented diagnostic solutions.
As a technology center, Leitat provides a unique positioning that combines scientific excellence with industrial translation.
Unlike traditional CROs, we operate at the intersection of research, innovation and industrial application, enabling companies to move from concept to validated solution within a collaborative R&D environment.
Our Strengths:
Our antibody platform is supported by advanced infrastructure and extensive research experience:
This ensures robust, reproducible and high-quality antibody development outcomes.
Behind every successful antibody development project is a multidisciplinary team of scientists with expertise spanning immunology, molecular biology, protein engineering and diagnostic assay development.
Our experts work collaboratively across every stage of the workflow, ensuring scientific excellence from discovery to validation.
Work with our scientific team to design, develop and validate antibodies tailored to your application needs. Let's discuss your antibody project now:
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